microRNA discovery by microarray analysis

MicroRNA are a class of highly evolutionarily conserved endogenous non coding small RNA with about 21 nucleotides in length (Ambros , 2001; Ambros et al ., 2003). Large number of microRNA  have been report in various plants species (Llave et al ., 2002b; Park et al ., 2002; Reinhart et al .,2002; Palatnik et al ., 2003; Bonnet et al ., 2004; Floyd and Bowman, 2004; Jones-Rhoades and Bartel, 2004; Sunkar and Zhu, 2004; Wang et al ., 2004a, 2004b; Adai et al ., 2005; Bedell et al ., 2005). Increasing evidence suggests that plant microRNA play vital roles in multiple essential biological processes, such as leaf morphogenesis and polarity (Plantinik et al .,2003), floral organ identity (Aukerman and Sakai, 2003; Chen 2004), phase changes from growth to generative growth (Aukerman and Sakai 2003 ; Chen 2004), virus  stress (Kasschau et al, 2003), mineral nutrient and dehydration stress (Sunjar and Zhu 2004).

LC Sciences provides a genome-wide microRNA (miRNA) expression profiling service using µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs. Standard arrays for mature miRNA of all species available in the latest version of the miRBase database (Release 17).Service is comprehensive and includes sample preparation of your total RNA sample, single or dual color labeling, hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, the analyzed data, representative and original images of the array, and raw data will be send to you. The in-depth data analysis may include multi-array normalization, t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.